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Cell Biology
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DNA Modifying Enzymes
T4 DNA Ligases
Hi-T4 DNA Ligase
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Hi-T4 DNA Ligase
Hi-T4 DNA Ligase from New England Biolabs
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Citations:
(5)
Supplier Page
Supplier Page from
New England Biolabs for
Hi-T4 DNA Ligase
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Hi-T4 DNA Ligase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M2622L
Quantity
100000 units
Applications
DNA Manipulation
Type
Enzymes
Temperature
-20 C
Brand/Trademark
New England Biolabs Inc (NEB)
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (5)
(1)
The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity
Veterinary Research
November 15, 2023
Min Zhang, Lilei Lv, Huaye Luo, Hongming Cai, Lingxue Yu, Yifeng Jiang, Fei Gao, Wu Tong, Liwei Li, Guoxin Li,
et al.
lentiviral vector pLV-EF1a-IRES-Hygro (Addgene, Cat#:85134) using a
Hi-T4 DNA Ligase
Kit (
NEB
, Cat#:
M2622S
). Similarly, the sequence encoding EGFP (enhanced green fluorescent protein) was amplified with primers
(2)
An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly
Cell Reports Methods
August 22, 2023
Masato Sorida, Roberto Bonasio
,
Thermo Scientific
,
SM1331
BsaI-HFv2
,
NEB
,
R3733S
BbsI-HF
,
NEB
,
R3539S
AgeI-HF
,
NEB
,
R3552S
BamHI-HF
,
NEB
,
R3136S
EcoRI-HF
,
NEB
,
R3101L
HindIII-HF
,
NEB
,
R3104S
NcoI-HF
,
NEB
,
R3193S
NotI-HF
,
NEB
,
R3189S
SalI-HF
,
NEB
,
R3138S
SpeI-HF
,
NEB
,
R3133S
XbaI
,
NEB
,
R0145S
XhoI
,
NEB
,
R0146S
XmaI
,
NEB
,
R0180S
KpnI-HF
,
NEB
,
R3142S
NdeI
,
NEB
,
R0111S
Smal,
NEB
,
R0141S
BbsI-HF
,
NEB
,
R3539M
T4 DNA Ligase
,
NEB
,
M0202L
Hi-T4 DNA Ligase
,
NEB
,
M2622S
100 mM ATP,
NEB
,
P0756S
NEBuilder HiFi DNA Assembly Master Mix
,
NEB
,
E2621
LB Broth
,
Fisher Scientific
,BP9723 Agar,Fisher
(3)
Improved USER cloning for TALE assembly and its application to base editing
PLoS One
August 4, 2023
Jizeng Zhou, Jiaowei Wang, Fangbing Chen, Zhenpeng Zhuang, Min Chen, Yang Yang, Xian Luo, Chengcheng Tang, Xiaoqing Zhou, Yue Chi,
et al.
assembled with
T4 DNA Ligase
(
New England Biolabs
, #
M0202
) or
Hi-T4 DNA Ligase
(
New England Biolabs
, #
M2622
). In constructing all the single-guide RNA (sgRNA), two complementary oligonucleotides were synthesized
(4)
Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftment
Leukemia
February 1, 2023
Wang X, Cooper S, Broxmeyer HE, Kapur R.
After annealing, double-strand oligos were ligated into the
shRNA
expression plasmid by T4 ligase (
NEB
,
M2622L
). Well-constructed vectors were sent to Genewiz (South Plainfield, NJ, USA) for
sequencing
.
Lentivirus
(5)
HRD1-mediated METTL14 degradation regulates m(6)A mRNA modification to suppress ER proteotoxic liver disease
Molecular cell
December 16, 2021
Wei J, Harada BT, Lu D, Ma R, Gao B, Xu Y, Montauti E, Mani N, Chaudhuri SM, Gregory S,
et al
.
M7449 Q5 HiFi DNA Polymerase,
New England Biolabs
,Cat#
M0491
T4 DNA ligase
,
New England Biolabs
,Cat#
M2622S
Complete proteinase
inhibitor
cocktail,Roche,Cat# 11697498001
Protein G Magnetic Beads
,
New England Biolabs
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Hi-T4 DNA Ligase from New England Biolabs
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